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cloning pcr amplified fragments  (Addgene inc)


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    Structured Review

    Addgene inc cloning pcr amplified fragments
    Cloning Pcr Amplified Fragments, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloning+pcr+amplified+fragments/pEGFPC1-hVAP-A+(Plasmid+%23104447)/bio_rxiv__2025__05__29__656564-286-20-25
    Average 92 stars, based on 9 article reviews
    cloning pcr amplified fragments - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Retroviral:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Expressing:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Mutagenesis:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Construct:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Cloning:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Polymerase Chain Reaction:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..

    Plasmid Preparation:

    Article Title: STARD3 coordinates Endoplasmic Reticulum-late endosome/lysosome contacts and organelle positioning through a GSK3-regulated phosphorylation switch
    Article Snippet: The GFP-MOSPD2 (WT Addgene plasmid # 186467; http://n2t.net/addgene:186467 ; RRID:Addgene_186467 and RD/LD mutant Addgene plasmid # 186468; http://n2t.net/addgene:186468 ; RRID:Addgene_186468), and Flag-tagged STARD3 expression vectors were previously described ( ; ). .. The retroviral expression vector pQCXIP encoding GFP-VAP-A (WT and KD/MD mutant) and GFP-VAP-B (WT and KD/MD mutant) were constructed by cloning PCR-amplified fragments from pEGFPC1-hVAP-A (Addgene plasmid # 104447), pEGFPC1-hVAP-A KD/MD (Addgene plasmid # 104449), pEGFPC1-hVAP-B (Addgene plasmid # 104448), and pEGFPC1-hVAP-B KD/MD (Addgene plasmid # 104450). ..



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